One Of The Few Human CCR7 Antibodies That Works Well

September 26, 2016

Immunology
Columbia University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-human CCR7 AF488

Catalog Number:

353206

I have tried many CCR7 antibodies and this is one of the few that works. Separation is ok, but when plotted against CD45RA easy to separate memory subsets.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human splenocytes

Primary Incubation

30 min on ice

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD Fortessa

Results Summary

Two images shown, can see from the one that separation by itself is just ok but when plotted against CD45RA can easily distinguish memory T cell subsets.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

It works

The Bad

Separation isn't the best

The Bottom Line

Use this antibody for CCR7

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