DNA Extraction From Biosolids

Durban University of Technology
IWWT
Post doctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

MO BIO Laboratories

Product Name:

PowerFecal® DNA Isolation Kit

Catalog Number:

12830-50

I am working on the development of molecular techniques for the detection of bacteria in the environmental samples. The PowerFecal® DNA Isolation Kit was used in extraction of total DNA from samples collected from wastewater treatment plants.

Experimental Design and Results Summary

Application

DNA Extraction

Starting Material

Samples from wastewater treatment plants

Protocol Overview

Protocol mentioned is easy and quick to perform.

Tips

Do not add starting material more than 0.25 grams per prep and try to remove residual Solution C5 completely.

Results Summary

Quality of DNA obtained was good.

Additional Notes

None

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Summary

The Good

Good quality DNA from samples high in PCR inhibitors

The Bad

Sometimes yield is low

The Bottom Line

Overall a good kit for fast and easy DNA extraction from complex matrices like fecal samples or biosolids

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