Good PLAU Antibody

Neurosurgery
Havard medical school
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotech

Product Name:

uPA Antibody (H-140)

Catalog Number:

sc-14019

This is an OK PLAU antibody. It gives you two bands probably for the cleaved chains of uPA. I am interested in different proteins that are secreted through extracellular vesicles in the extracellular space.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysates from human glioma cell lines

Primary Incubation

5% milk, 4°C, overnight, shaker

Blocking Agent

5% milk, 1h, RT, shaker,

Secondary Incubation

5% milk, 1h, RT, shaker,

Tertiary Incubation

N/A

Detection

BIO RAD Imager

Results Summary

Good PLAU (uPA) antibody. Gives you two brand depending if uPA is cleaved

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to use. detects multiple chains

The Bad

Different bands can be confusing.

The Bottom Line

Good and easy PLAU (uPA) antibody to use

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