BD Biosciences
Anti-RIP1 antibody
610458
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Used this antibody for a western blot to detect RIP1 (RIPK1) protein levels in mouse lung. The mice whole lung were harvested, homogenized, lysed in Pierce IP lysis buffer in the presence of protease inhibitor cocktail (Roche) and phosphatase inhibitor cocktail (Sigma). After 30 minute incubation on ice, the protein lysates were extracted by centrifuging at 13200 rpm for 10 minutes on a desktop centrifuge at 4 degree. The protein lysates were then subject to western blot analysis to determine the expression level of RIP1.
Western Blot
Mouse whole lung lysate
1:1000
Li-Cor Odyssey® Blocking Buffer (TBS)
Li-Cor IRDye 680 Goat anti-mouse IgG
1:15000
Near-Infrared Fluorescence Imaging (NIR)
The antibody works well and detects specific band for mouse RIP1 (about 70 kDa).
None
Specific band detected.
None.
This antibody works well and is the most well-used antibody of mouse RIP1 (RIPK1) studies.