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Date Published
Recombinant Human B7-H3 Fc Chimera Alexa Fluor® 488 From R&D Systems Works but Does Not Provide the Best Signal
(1)
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R&D Systems, a Bio-Techne Brand
December 24, 2023
I used this antibody to detect the % of the binding of my T cell to this fluorophore-conjugated B7H3. I found that this antibody worked but ...
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Easiest ROCK Inhibitor From Gibco for Thawing and Passing IPSCs
(1)
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Thermo Fisher Scientific
December 24, 2023
I use this post-thaw or if I pass with accutase to reduce cell death. I prefer it to other ROCK inhibitors because it comes premade and is ...
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Excellent B Cell Purification With the Kit From Miltenyi Biotec
(1)
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Miltenyi Biotec
December 24, 2023
We are studying the pathways of B cell activation leading to different antibody-producing cell differentiation. For this, we test different ...
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Anti-Mouse CXCR6 BV421 Antibody From BioLegend Stains Well
(1)
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BioLegend
December 23, 2023
We use this antibody to stain for CXCR6 on ILCs in mouse lymphoid organs. It can be dim and hard to distinguish positive from negative, but ...
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PE/Fire™ 640 Anti-Human CD183 (CXCR3) Antibody With Distinct Staining
(1)
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BioLegend
December 23, 2023
This antibody has been used to optimize flow cytometry-based immunophenotyping of PBMC samples. The results have not yet been published. ...
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Great Antibody From BD Biosciences With Good Staining
(1)
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December 23, 2023
We always use this antibody to stain for T cells in the mouse lymphoid organs. It can be dim sometimes in the tissues so we usually use it ...
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Superb Reagent Used for RNA Fragmentation
(1)
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Thermo Fisher Scientific
December 23, 2023
This product works great. Needs Heat blocks and ice. Able to obtain desired fragments based on incubation times.
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Decent Anti-Mouse CD3 FITC Antibody From Tonbo Bioscience
(1)
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Tonbo Biosciences
December 23, 2023
I used this antibody to stain T cells in the mouse lymph nodes for immunofluorescence. It was a little dim but still looked okay if you ...
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PE/Dazzle™ 594 Anti-Human CD20 Antibody From BioLegend Works Well
(1)
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BioLegend
December 23, 2023
This antibody has been used to optimize flow cytometry-based immunophenotyping of PBMC samples. The results have not yet been published. ...
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Bright and Robust Anti-Human CTLA-4 Staining
(1)
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December 23, 2023
Our research aims to understand how some CD4 T cells become autoreactive in melanoma patients treated with checkpoint inhibitors. In this ...
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Easy and Effective ELISA Kit From RayBioTech
(1)
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RayBiotech
December 23, 2023
In an attempt to detect LAG3 in cell culture supernatants, this ELISA kit provided an accurate and reliable method for detection. The ...
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Quick and Easy Detection of SEAP Using Quanti-Blue Solution
(1)
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December 23, 2023
My work requires using cell lines with stable reporters that secrete the SEAP (secreted embryonic alkaline phosphatase) protein. QuantiBlue ...
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Very Good Brilliant Violet 711™ Anti-Human CD185 (CXCR5) Antibody From BioLegend
(1)
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BioLegend
December 23, 2023
This antibody has been used to optimize flow cytometry-based immunophenotyping of PBMC samples. The results have not yet been published. ...
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Good PCR Purification Kit From Qiagen
(1)
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December 22, 2023
This kit is used daily in my lab, it allows purification of PCR products in a fast and easy way. I follow the supplier's protocol with the ...
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Super SuperSignal Western Blot Enhancer From Thermo Fisher
(1)
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Thermo Fisher Scientific
December 22, 2023
We used regularly our protein assays and analysis, protein biology in western bottling. very small volume for A and B solution perfect ...
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Microplates Suitable for Multiple Applications
(1)
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Corning Life Sciences
December 22, 2023
This product was a little difficult for me to find, but I like it a lot now. I used the black colored plates for the luciferase assay.
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RORgt Antibody From eBioscience Gives Clear Intra-Nuclear Staining
(1)
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Thermo Fisher Scientific
December 22, 2023
When used for flow cytometry on intestinal cells, there will be some background. But, the RORgt cell population is clear.
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Excellent Antibody for CD3 From BD Biosciences
(1)
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December 22, 2023
This antibody gives good separation of CD3-positive cells in mouse intestines. The signal is bright and specific.
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Easy to Use Stirring Hotplate From Thermo Fisher
(1)
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Thermo Fisher Scientific
December 22, 2023
My research involves creating nanoparticles. In the formulation I need to have an overnight dialysis of my particles with a stirring rod. ...
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Anti-Mouse CD4 Antibody From BioLegend Shows Great Staining
(1)
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BioLegend
December 22, 2023
This antibody gives great CD4 staining in mouse intestine cells. The positive signal is strong and specific.
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VCAM1 From Abcam for Rat Samples
(1)
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Abcam
December 22, 2023
VCAM1 is a cell adhesion molecule that regulates inflammation-associated vascular adhesion. Our goal was to detect VCAM1 in the brains of ...
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Excellent Attachment Factor for Cells
(1)
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Thermo Fisher Scientific
December 22, 2023
In our cell culture used mostly in attachment factor solution (AFS) is a sterile-filtered solution of attachment factor. It is used as a ...
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Reliable Beads for Compensation Using Amine Dyes
(1)
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Thermo Fisher Scientific
December 22, 2023
We differentiate dendritic cells from CD14+ PBMCs with the goal of assessing dendritic cell maturation/activation by flow cytometry after ...
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Great Anti Human IL-1 Beta Antibody
(1)
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R&D Systems, a Bio-Techne Brand
December 21, 2023
IL-1ß are multifunctional cytokines and play pivotal roles in inflammation and infection. I used this antibody to check our treatment effect...
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Nice Anti-Mouse F4/80 antibody
(1)
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December 21, 2023
I used this anti-mouse F4/80 antibody in several of my projects for macrophage staining. It worked well both in vitro and in vivo.
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Strong, Sturdy and Sterile Polypropylene Centrifuge Tubes From Fisher Scientific
(1)
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Thermo Fisher Scientific
December 21, 2023
We use these tubes during sterile cell experiments. They are very sturdy and never crack (which would cause contamination). They work great ...
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Not a Great Experience with the Human Estradiol ELISA Kit
(1)
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BosterBio
December 21, 2023
This kit was used to assess saliva samples sent through the mail to provide at-home tests for patients. The goal was to provide accurate and...
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Good CD5 Antibody for T- Cells Detection
(1)
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December 21, 2023
The CD5 antibody can be used to detect human iPSC/ESC-derived T cells with ease. The antibody shows a clear separation between positive and ...
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Bright and Specific CD8 T Cell Tetramer for Identification of Spike Protein Specific CD8+ T Cells
(1)
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December 21, 2023
The APC-conjugated S539 Tetramer has been working well for identifying CD8+ T cells that respond to the Spike protein from SARS-CoV-2 in ...
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Nice Anti-human IFN-gamma Antibody
(1)
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R&D Systems, a Bio-Techne Brand
December 21, 2023
Interferon-? (IFN?) is an important mediator of cellular immune responses. I used this anti-human IFN-gamma Antibody to test IFN-gamma ...
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Great Serum-Free Dendritic Cell Medium From CellGenix
(1)
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CellGenix GmbH
December 21, 2023
The goal of my research is to culture primary human dendritic cells from CD14+ cells, and use them in downstream experiments that promote DC...
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Excellent Viability Dye From Logos Biosystems for Quick Viability Measurement
(1)
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Logos Biosystems
December 21, 2023
This research aimed to assess the viability of cells after treatment with photothermal therapy at different doses.
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Excellent Reagent From AmericalBio to Prepare Blocking Buffer for ELISA
(1)
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December 21, 2023
To detect antibodies against human common cold viruses in pre-pandemic serum samples, I have used non-fat dry milk from americanBio to ...
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Ideal Reagent for Antibiotic Selection
(1)
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Thermo Fisher Scientific
December 20, 2023
Hygromycin B is a water-soluble antibiotic purified from the bacterium Streptomyces hygroscopicus. Hygromycin B acts by binding to the 80S ...
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Human T4 ELISA Kit from Boster
(1)
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BosterBio
December 20, 2023
The goal of this experiment is to quantify the levels of human T4 in dried blood spots from at-home test kits. This assay in combination ...
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Simple and Effective Tool for Quick Cell Counting
(1)
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Logos Biosystems
December 19, 2023
We use this cell counter for basic cell culture for a variety of cells and cell lines, including cancer cell lines and human donor-derived ...
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Bright and Specific IL-2 Antibody from BioLegend
(1)
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BioLegend
December 18, 2023
Functional characterisation of T cell. Human PBMC were stimulated with PMA/onomycin for 5 hours in the presence of BFA. Cells were then ...
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Wonderful for Growing MDA-MB-231 Cells
(1)
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December 18, 2023
This media allows for mda-mb-231 cells to grow fast and well. These cells are tricky so I am happy I found this media that works.
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A Good Brilliant Violet 711 anti-mouse NK1.1 Antibody
(1)
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BioLegend
December 18, 2023
This antibody provides a good NK1.1 mouse staining on lymphocytes and an acceptable background. It allows good detection of mouse NK1.1-...
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Specific Neurofilament Light Chain Antibody
(1)
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MilliporeSigma
December 18, 2023
The specific goal of this experiment was to evaluate neurofilament light chain expression changes in the course of diseases. This antibody ...
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Specific IL-4 Antibody from BioLegend
(1)
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BioLegend
December 13, 2023
Functional characterisation of T cell. Human PBMC were stimulated with PMA/onomycin for 5 hours in the presence of BFA. Cells were then ...
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Electronic 12 Channel Pipette
(1)
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Sartorius
December 13, 2023
We use this pipette for dispensing reagents into microtiter plates for ELISpot and ELISA experiments.
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Good Reagent, Slight Toxicity Depending on Dilution
(1)
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December 13, 2023
Used to assay T cell proliferation in response to stimulus. The reagent works well and I can see nice peaks representing division. This ...
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Vimentin Antibody from Thermo Fisher Scientific for Western Blot
(1)
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Thermo Fisher Scientific
December 13, 2023
We study multiple pathological processes in ocular diseases. The specific goal of this experiment was to evaluate vimentin protein ...
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Efficient Labelled Primary Ki-67 Antibody for Assessing Cell Proliferation
(1)
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Cell Signaling Technology
December 13, 2023
Endothelial cells were treated with extracellular vesicles (EVs) derived from various cell types. Ki-67 staining was used to assess the ...
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Decent BioLegend CCR6 staining on mouse lymphocytes
(1)
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BioLegend
December 13, 2023
Decent staining of CCR6-positive populations in mouse MC38 tumor-infiltrating lymphocytes. It is a useful antibody to detect TH17-like cells
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Alexa 594-conjugated Anti-mouse Antibody for Bright Fluorescence Detection
(1)
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Thermo Fisher Scientific
December 13, 2023
This secondary chicken antibody against mouse IgG (H+L) conjugated with Alexa 594 is an easy-to-use option to detect mouse antibody labelled...
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Great BCA Protein Assay Kits
(1)
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Thermo Fisher Scientific
December 13, 2023
I have been using this BCA protein assay kit to measure protein concentration for more than 7 years. The results are very accurate.
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Multi-dispenser Pipette from Starlab
(1)
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December 13, 2023
RepeatOne® is a hand-held device for repetitive pipetting. Up to 48 pipettings of the same sample can be made from a single filling.
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Provides Satisfactory Staining for CD45.2+ Populations
(1)
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BioLegend
December 12, 2023
I utilized this antibody to detect what percentage of the cells I had isolated from my mouse tumors were CD45.2+. It worked as intended to ...
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