Invitrogen™
beta Tubulin Loading Control Monoclonal Antibody (BT7R)
MA5-16308
BT7R
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As a researcher, I find Western blotting to be a powerful technique for investigating protein stability, interactions, and expression levels. To ensure the accuracy of my results, incorporating a loading control is a critical step in my experimental workflow. For this purpose, I consistently rely on the beta-tubulin antibody due to its stability and widespread presence in eukaryotic cells. Selecting a reliable and species-specific beta-tubulin antibody is essential to avoid any potential cross-reactivity issues. I always validate the antibody under my experimental conditions to ensure it accurately detects beta-tubulin. During gel loading, I carefully load an appropriate amount of total protein, within the linear range of detection, to obtain reliable results. Analyzing the beta-tubulin bands alongside my target protein bands enables me to normalize the data and account for any variations in protein loading.
Western blot
Cell extract
1:5000 in blocking buffer (5% milk in TBST) for 16 hr at 4degC
5% milk in TBST
Goat anti-mouse IgG HRP at 1:5000 dilution in blocking agent for 1 hr at room temperature
N/A
Using ECL reagent and exposing film for 2 sec
The uniform loading of beta-tubulin across different samples on the blot confirms the effectiveness of using beta-tubulin as a loading control in my Western blot experiments. This result validates that equal amounts of protein have been loaded in each lane, providing a reliable basis for comparing and analyzing the target proteins' stability, interactions, and expression levels under different experimental conditions.
Good antibody with minimal cross-reactivity.
None.
Beta-tubulin antibody as my loading control ensures accurate protein analysis in Western blotting.