Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit From Thermo Fisher is Better Than BrdU

January 25, 2024

BlueskyReddit
UNC Chapel Hill

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Company:

Thermo Fisher

Product Name:

Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit

Catalog Number:

C10420

The Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells. Newly synthesized DNA is analyzed using the 488 nm laser of the flow cytometer.

Experimental Design and Results Summary

Application

Flow cytometry

Starting Material

Cells

Tips

I use the dilution as recommended

Results Summary

Many advantages over others: Accurate and superior results compared to BrdU assays. Fast- results in as little as 90 minutes. Economical- more assays per kit. By increasing the number of assays per kit, the Click-IT™ EdU Alexa Fluor 488 Flow Cytometry Assay Kit is less expensive than the traditional BrdU assays making them ideal for large experiments.

DOI or PMID #

N/A

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Summary

The Good

Works well with all cell lines.

The Bad

Can't think of anything bad.

The Bottom Line

The Click-iT EdU protocol is based on the aldehyde fixation and detergent permeabilization steps for immunohistochemical antibody labeling, but EdU is compatible with other fixation/permeabilization agents including saponin and methanol. In just five steps you’ll be ready to analyze your cell proliferation data:• Treat cells with EdUM• Fix and permeabilize cells• Detect S-phase cells with Click-iT™ detection cocktail for 30 min• Wash once• Analyze.

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