This Antibody From BioLegend Clearly Stains CD8a+ T Cells in Primary Cells

January 26, 2024

BlueskyReddit
Immunology
George Washington University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse CD8a Antibody

Catalog Number:

100708

Clone Number:

53-6.7

I regularly use this antibody in my basic immune cell panel to identify CD8a subpopulations within my CD3+ T cells. Especially because it is conjugated to PE, the signal is very bright and clear to distinguish.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse primary T cells

Primary Incubation

1:100 at 4C for 30 minutes in the dark

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

I was able to utilize this antibody to identify CD8 subsets within my mouse Pan T cells. The population was distinct and clear enough to quantify this population.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright, and should accessible for most cytometers.

The Bad

Requires titration.

The Bottom Line

For a CD8a anti-mouse antibody, this is a sufficient antibody for your flow cytometry panel. Since it is conjugated to PE, it should be compatible for most cytometers and maintains good signal even when fixed.

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