Invitrogen™
Actin Monoclonal Antibody
MA1-744
mAbGEa
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By consistently using the stable and species-specific actin antibody as my loading control, I ensure accurate protein analysis in Western blotting, allowing me to confidently investigate protein stability, interactions, and expression levels in my research. The reliable detection of actin in each experiment validates equal protein loading, enabling meaningful comparisons across different samples and experimental conditions. This systematic approach ensures the robustness of my findings and enhances the overall reliability of my Western blot data.
Western blot
Cell extract
1:10000 in blocking buffer (5% milk in TBST) for 16 hr at 4 degrees C
5% milk in TBST
Goat anti-mouse IgG HRP at 1:5000 dilution in blocking agent for 1 hr at room temperature.
NA
Using ECL reagent and exposing film for 2 sec
Having consistent actin expression across different samples on the blot confirms the successful use of actin as a loading control in my Western blot experiments. This ensures equal protein loading in each lane, providing a reliable foundation for comparing and analyzing the stability, interactions, and expression levels of target proteins under various experimental conditions. The uniform actin bands empower me to confidently normalize my data, enabling meaningful and accurate conclusions to be drawn from my research findings.
Good antibody with minimal cross-reactivity.
None.
The consistent expression of actin across different samples on the blot validates the reliability of using the actin antibody as a loading control in Western blotting experiments.