Decent Anti-Mouse CD80 Antibody From eBioscience

January 16, 2024

BlueskyReddit
Medicine
Weill Cornell Medicine
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-mouse CD80 (B7-1) Monoclonal Antibody FITC

Catalog Number:

11-0801-82

Clone Number:

16-10A1

This antibody provides a good CD80 mouse staining on CD45-positive immune cells and an acceptable background. It allows good detection of mouse CD80-positive immune cells.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse caecum cells

Primary Incubation

1/200 in PBS

Blocking Agent

Fc Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR Fortessa II

Results Summary

I use this antibody for detecting CD80-positive immune cells among CD45-positive immune cells from mouse caecum tissue. The staining was convincing and specific to perform proper gating strategy.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good and specific staining.

The Bad

Require antibody titration to improve the staining at the best.

The Bottom Line

Good staining of CD80 in mouse CD45-positive cells from mouse caecum tissue. Great for detecting CD80-positive intestinal immune cells.

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