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Excellent DMEM Medium for Cell Culture From Corning
(1)
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Corning Life Sciences
December 07, 2023
Our experiment for cell culture medium used regularly in Dulbecco’s Modified Eagle’s Medium contains four times the concentration of amino ...
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A Must Have in Your Flow Cytometry Panel
(1)
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Thermo Fisher Scientific
December 07, 2023
I used the UltraComp beads to serve as my single color controls for flow cytometry. These beads were extremely convenient to use since they ...
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Great Anti-Human CD14 Antibody for Flow From BioLegend
(1)
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BioLegend
December 07, 2023
To detect and quantify the difference in the number of monocytes in human PBMCs collected from children and adult participants, I have used ...
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Excellent Basal Growth Fibroblast Medium From PromoCell
(1)
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December 07, 2023
The study of the Fibroblast basal Growth Medium for use of mouse tissues fibroblast cell isolation showed that the growth of the cells was ...
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3ZZ Oprm1 Probe From ACD for BaseScope-ISH in Mouse Samples
(1)
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Advanced Cell Diagnostics
December 07, 2023
We used the Oprm1 3ZZ probe to target Oprm1 mRNA (GenBank accession NM_001039652.2; nucleotides 845–976) in mouse fresh frozen brain ...
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Easy to Use RNA/Short DNA Fragment Clean Up Kit From Zymo Research
(1)
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December 07, 2023
Our lab is interested in using cutting-edge technologies to better understand the mechanisms responsible for immune self-tolerance and self-...
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Effectiveness of This Reagent From Quality Biological Depends on Your Sample
(1)
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Quality Biological, Inc.
December 06, 2023
I used this buffer to lyse the red blood cells that were in my digested tumor tissue. Depending on the size of the tumor, you may need to ...
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Excellent Cell Digestion Buffer from Promega
(1)
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Promega
December 06, 2023
Our cell culture experiments use lysis buffers to disrupt the cell membrane. Lysis buffers break the cell membrane by changing the pH. ...
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Great Counterstaining for Immunofluorescence Assays
(1)
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Thermo Fisher Scientific
December 06, 2023
We are performing a cell-based flavivirus infection assay to determine the EC50 of different viruses against a compound. To generate a dose-...
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Fast PCR Mix From Vazyme
(1)
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December 06, 2023
We use this PCR master mix to amplify genes from plasmids and cellular chromosomes from cell culture or mouse tissues. This PCR mix is easy ...
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Great Occludin Antibody From Abcam for IHC-IF
(1)
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Abcam
December 06, 2023
The tight junction occludin can be detected in both endothelial and epithelial cells. We aimed to detect Occludin in the brains of rats and ...
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Relatively Dim CD45.2 Antibody for Flow Cytometry
(1)
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BioLegend
December 06, 2023
The BV510 CD45 antibody works well for identifying CD45+ immune cells from cell populations isolated from mouse lungs and bronchoalveolar ...
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Excellent Trypsin Inhibitor From Thermo Fisher
(1)
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Thermo Fisher Scientific
December 06, 2023
In our cell culture, Trypsin inhibitors are used in cell culture experiments to further inhibit tryptic activity during cell dissociation to...
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Antibody From BioLegend Works as Intended With Bright Signal
(1)
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BioLegend
December 05, 2023
I needed to identify the T cells that were adoptively transferred from a mouse with a CD45.1 background to a wild-type C57BL/6 mice. Using ...
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Viability Kit From BioLegend Requires Titration as It Provides Bright Signal
(1)
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BioLegend
December 05, 2023
I have used other Zombie Viability dyes in the past and here have Zombie Aqua to stain for the viability of my tumor cells. Using Zombie ...
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Great BCA Protein Assay Kit From Thermo Fisher
(1)
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Thermo Fisher Scientific
December 05, 2023
We have been using this BCA protein kit for protein concentration measurement for over 8 years. It worked very well.
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Great GAPDH Antibody From ThermoFisher Scientific for Western Blot
(1)
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Thermo Fisher Scientific
December 05, 2023
We study understanding multiple pathological processes in ocular diseases. The specific goal of this experiment was to evaluate GAPDH ...
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Decent CD19 Antibody From BD Biosciences
(1)
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December 05, 2023
A good antibody that can work very well. There can be some difficulty with compensation, but overall the antibody is okay.
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A Good PE Anti-Mouse TCR γ/δ Antibody From BioLegend
(1)
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BioLegend
December 05, 2023
When used for flow cytometry of intestinal lymphocytes, the detection of TCR ?/d positive cells is good and allow separation from ...
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Great Media From Cytiva for J774 Cells
(1)
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Cytiva Life Sciences
November 30, 2023
This media is grown for J774 cells which are extremely specific in the type of way they like to grow. This media has the right combination ...
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Superb Human Progesterone ELISA Kit From Boster
(1)
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BosterBio
November 30, 2023
The goal of this experiment was to quantify the levels of progesterone in saliva for at-home test kits. In combination with other kits, we ...
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This Antibody From BD Biosciences is Ideal for T Cells Detection
(1)
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Pharmingen
November 30, 2023
The CD7 antibody can be used to detect early T cell progenitors that are CD7 and CD5 positive. The antibody is very easy to use and clearly ...
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Brilliant Violet 421™ anti-human IFN-γ Antibody From BioLegend is Worth it for the Price
(1)
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BioLegend
November 30, 2023
Functional characterization of T cell. Human PBMCs were stimulated with PMA/ionomycin for 5 hours in the presence of BFA. Cells were then ...
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The Human XL Cytokine Luminex Performance Panel Premixed Kit is a Simpler Alternative to Performing Multiple ELISAs
(1)
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R&D Systems, a Bio-Techne Brand
November 30, 2023
In this study, we treated U87 glioblastoma cells with different cell treatment methods to determine the effect of each treatment on U87 ...
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Great Antibody Concentrator Kit for Oligo/Fluorophore Conjugation
(1)
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Abcam
November 30, 2023
Our lab is interested in using cutting-edge technologies to better understand the mechanisms responsible for immune self-tolerance and self-...
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Anti-Mouse/Human CD11b Antibody Shows Excellent Staining
(1)
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BioLegend
November 30, 2023
This antibody provides a good signal for CD11b positive cells in mouse intestines. The signal is strong and specific.
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Great Dishes From Thermo Fisher for Culturing of Cells That Float
(1)
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Thermo Fisher Scientific
November 29, 2023
This type of dish is perfect for J774 cells that are grown in my lab. They are floating cells and these dishes allow them to grow and expand...
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Reliable Zombie NIR™ Fixable Viability Kit From BioLegend
(1)
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BioLegend
November 29, 2023
The Zombie NIR viability kit works well for identifying dead cells from cell suspensions isolated from mouse lungs, spleen, and lymph nodes....
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Bright and Specific IL-17A Antibody From BioLegend
(1)
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BioLegend
November 29, 2023
Functional characterization of T cell. Human PBMCs were stimulated with PMA/ionomycin for 5 hours in the presence of BFA. Cells were then ...
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Anti-Human CD16-FITC Antibody From BioLegend Has Good Signal
(1)
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BioLegend
November 29, 2023
To detect and quantify the frequency of monocytes in human PBMCs collected from diseased and healthy human subjects, I have included this ...
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Good Anti-Human HLA-DR-BV421 Antibody From BioLegend Ideal for Flow
(1)
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BioLegend
November 29, 2023
To check the increase in the frequency of dendritic cells in human PBMCs collected from respiratory virus-infected patients, I have used ...
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Anti-Mouse TNF-Alpha Antibody From BioLegend for Flow Cytometric Detection of Cytokine Production
(1)
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BioLegend
November 29, 2023
The BV785 TNF-alpha antibody works well for identifying cytokine-producing activated CD4 or CD8 T cells isolated from mouse lungs, spleen, ...
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Fast and Wonderful ChemiDoc Imaging System From Bio-Rad for Western Blotting
(1)
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Bio-Rad
November 28, 2023
This is a great machine that gives great Western Blotting results. I like the method through which it does chemiluminescence.
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Excellent Anti-Human CD45-FITC From BioLegend for Flow
(1)
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BioLegend
November 28, 2023
For immunophenotyping of different types of cells present in human blood, I used an anti-human CD45-FITC antibody in my master mix to detect...
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Probumin Bovine Serum Albumin From Millipore Works Very Well in IHC
(1)
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MilliporeSigma
November 28, 2023
There is no need to pay high prices for expensive counterparts. This albumin from Millipore works very well in all stages of ...
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Anti-Mouse IFN-Gamma Antibody From BioLegend for the Flow Cytometric Detection of Cytokine Production
(1)
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BioLegend
November 28, 2023
The BV711 IFN-gamma antibody works well for identifying cytokine-producing activated CD4 or CD8 T cells isolated from mouse lungs, spleen, ...
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My Favorite Flasks From Geneseesci
(1)
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November 28, 2023
I have used many flasks and cell culture dishes. this is my favorite and I had to write this review. I have sensitive cells that I culture ...
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Smart Stack Pipet Tips Are Great Space Savers
(1)
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Thomas Scientific
November 28, 2023
These 200uL pipets work great and save lots of space since they stack on top of one another. They are great for multichannel pipets since ...
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Best Option for T-Bet Staining in Human
(1)
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Thermo Fisher Scientific
November 22, 2023
Our research aims to understand how some CD4 T cells become autoreactive in melanoma patients treated with check point inhibitors. In this...
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Excellent Anti-Mouse IL17A Antibody From Thermo Fisher
(1)
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Thermo Fisher Scientific
November 20, 2023
Bright and reliable anti-IL17a antibody at 1:200 dilution on LPMCs after restimulation with PMA/Ionomycin.
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Very Good Fixable Live/Dead Cell Detection Kit By Invitrogen
(1)
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Thermo Fisher Scientific
November 20, 2023
This stain has been used when optimizing flow cytometry-based immunophenotyping of PBMC samples. The results have not yet been published. ...
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Excellent Anti-Mouse IFNg Antibody From BD Biosciences
(1)
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November 20, 2023
Excellent staining result at 1:200 on LPMCs with eBioscience Fix/Perm kit after restimulation with PMA/Ionomycin.
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Very Good PerCP/Fire™ 806 Anti-Human IgD Antibody From BioLegend
(1)
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BioLegend
November 20, 2023
This antibody has been used to optimize flow cytometry-based immunophenotyping of PBMC samples. The results have not yet been published. ...
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Master Mix From New England Biolabs for Easy Plasmid Generation
(1)
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New England Biolabs
November 20, 2023
The NEBuilder system from New England Biolabs is a very convenient tool for building your plasmids. With specific primers created with an ...
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Excellent Antibody From Agilent for Canine Tissues
(1)
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Agilent Technologies
November 17, 2023
This antibody, specific to human tissues, reacts perfectly with high dilution in dog tissue by cross-reacting. Unfortunately, it does not ...
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Dependable Cell Dissociation Trypsin-EDTA Solution From Thermo Fisher
(1)
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Thermo Fisher Scientific
November 17, 2023
We routinely maintained various mammalian cell lines to test our hypothesis. I always use this trypsin-EDTA solution for dissociation and ...
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Very Good Spark Red™ 718 Anti-Human CD56 (NCAM) Antibody From BioLegend
(1)
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BioLegend
November 17, 2023
This antibody has been used to optimize flow cytometry-based immunophenotyping of PBMC samples. The results have not yet been published. ...
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Great Experience Using the BioTek Cytation 5 From Agilent
(1)
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Agilent Technologies
November 17, 2023
My goal was to understand interactions between cells and nanoparticles, and the biological effect of those nanoparticles on cancer and non-...
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Normal Tissue Culture Flask From Fisher Scientific
(1)
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Thermo Fisher Scientific
November 17, 2023
This is a great tissue culture flask that allows for many cells to grow continuously. I see that especially my THP1 cells like this type of ...
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Reliable Antibiotic Solution for Cell Culture From Thermo Fisher
(1)
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Thermo Fisher Scientific
November 17, 2023
We routinely do in-vitro studies to test our hypothesis in mammalian cell lines. To maintain the aseptic culture conditions, I always use ...
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