Unspecific ICC Staining of NPAS3 Abs From ProSci

SUNY UB
Research Scientist

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Company:

ProSci

Product Name:

NPAS3 Antibody

Catalog Number:

4107

Clone Number:

NPAS3 is a member of the basic helix-loop-helix PAS domain class of transcription factors expressed in the brain. NPAS3 is a replicated genetic risk factor for psychiatric disorders. A knockout mouse model exhibits behavioral and adult neurogenesis deficits consistent with human illness. We used the NPAS3 Abs to perform ICC in primary astrocyte cell cultures to validate the KO model.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Primary astrocyte cells cultures

Primary Incubation

1:100, overnight at +4 degrees C

Blocking Agent

Goat serum

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Zeiss AxioImager 2 fluorescent microscope equipped with an AxioCam 702 digital camera and ZEN software 2.6 version.

Results Summary

We used these NPAS3 Abs for validation our NPAS3 KO mouse model. We performed ICC with primary astrocyte cells cultures isolated from newborn pups. - We didn’t see the specific nucleus staining with anti-NPAS3 Abs;- We didn’t see the difference in staining between different genotypes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

N/A

The Bad

Unspecific staining.

The Bottom Line

Not recommend these NPAS3 Abs.

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