High Quality Total RNA Isolation With the RNeasy Mini Kit From Qiagen

University of South Florida
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Qiagen

Product Name:

RNeasy Mini Kit (50)

Catalog Number:

74104

The major focus of our research is to determine the significance of tubuloglomerular feedback (TGF) responsiveness in physiological and pathological conditions such as acute and chronic kidney injury. We utilize in vivo and in vitro sophisticated techniques such as microperfusion, micropuncture, fluorescence imaging, laser capture microdissection, molecular biology, cell biology, whole animal physiology, and transgenic animal models.

My research focuses on CRISPR/ Cas-9 drug delivery for treating HIV reservoirs in the brain. I frequently use this kit for isolating RNA for my molecular experiments.

Experimental Design and Results Summary

Application

Total RNA isolation

Starting Material

Kidney samples ( from rat and mice)

Tips

Easy to use

Results Summary

By using this kit, we were able to isolate the RNA and perform further RTPCR and gene expression studies. Thus, confirming the severity of kidney injury. This kit provides maximum sensitivity with precious samples in quantitative gene expression analyses and highly reproducible results for sensitive data.

DOI or PMID #

N/A

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Summary

The Good

Reliable and reproducible.

The Bad

None.

The Bottom Line

Highly recommended.

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