LS Bio
Human Neurogranin ELISA kit
LS-F33235
Info:Supplier Page
The ELISA kit for human neurogranin is intended for samples such as plasma, serum, and tissue homogenates. However, we used the kit for exosomes in solution (derived from human plasma), and the performance was good. The only adjustment made was the absence of the higher concentration in the curve (5000pg/mL), which I omitted as it was thought not necessary for exosomes (hardly will get that concentration). The kit is easy to optimize, and consistent with a range of different sample preparation.
ELISA (protein concentration)
Human Plasma; Total Exosomes; and brain-derived Exosomes
The kit seems to be suitable for different types of samples.
I used the kit to test the neurogranin concentration in exosomes derived from Human plasma, at different ages. At the same time, I tested with some immunopurified samples (neuronal-derived exosomes, such as those NCAM+) and test their neurogranin concentration. The results were consistent across my samples and were aligned with the literature in the topic. It seems that aging affects neurogranin concentration in exosomes in a negative correlation (as age increase, neurogranin concentration decrease in plasma).
Reliable, has a good signal, consistent and easy to use.
None.
Exceeded my expectations, once were suitable and reliable for samples such exosomes.