High-Quality COX10 Antibody From Proteintech

January 19, 2024

Physiology and Biophysics
State University of New York at Buffalo
Postdoctoral Researcher

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Company:

Proteintech

Product Name:

COX10 antibody

Catalog Number:

10611-2-AP

Clone Number:

Polyclonal

COX10 antibody from Proteintech was used to assess the expression of cytochrome c oxidase in isolated mouse astrocytes via Western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse; ex vivo astrocytes immunomagnetically isolated from the brain.

Primary Incubation

COX10 Polyclonal antibody (1:1000, Proteintech, 10611-2-AP); overnight incubation at 4 °C on shaker.

Blocking Agent

TBST containing 5% non-fat dry milk (Cell Signaling Technology, #9999).

Secondary Incubation

HRP-conjugated anti-rabbit secondary antibody (1:1000, NA934, Cytiva); 2 hour incubation at room temperature.

Tertiary Incubation

N/A

Detection

The immunoblots were visualized on Blu-Ray autoradiography films (NDA8803, Next Day Science) using Super Signal West Pico Chemiluminescent Substrate (34080, Thermo Fisher Scientific).

Results Summary

The COX10 antibody demonstrates exceptional specificity, yielding precise results without the presence of extra bands. Its reliability is underscored by consistent performance across experiments. The antibody has been validated using COX10 knockout samples (astrocytes isolated from Aldh1l1-Cre/ERT2::Cox10 fl/+ mouse brains), ensuring that the results obtained truly reflect the targeted protein's expression.

DOI or PMID #

35275429

Additional Notes

The antibody works well in a 1:1000 dilution.

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Summary

The Good

Exceptional specificity, which translates into clean and precise results.

The Bad

Relatively high price.

The Bottom Line

The COX10 antibody from Proteintech offers outstanding specificity, ensuring precise results without unwanted bands, while maintaining a consistent and reliable performance across experiments.

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