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Date Published
Chemoattractant on TRPM4 Expression
(1)
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Thermo Fisher Scientific
October 13, 2020
Here we checked the time course effect of TRPM4 expression by chemoattractant.
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Great Histone H3 (D1H2) for Western Blot
(1)
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Cell Signaling Technology
October 13, 2020
I used this antibody as a loading control for nuclear fraction of THP1 cell samples treated with different compounds.
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Great ECL Substrate
(1)
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Cell Signaling Technology
October 12, 2020
I use this reagent to develop my Western blots. Here I am showing the blot of Beta actin developed by SignalFire ECL reagent.
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Nice Bax (D2E11) Rabbit mAb for Western Blot
(1)
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Cell Signaling Technology
October 12, 2020
I determined the levels of Bax protein as a part of apoptosis examination in THP1 cells treated with different compounds.
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Great Secondary Antibody for Western Blot
(1)
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Cell Signaling Technology
October 12, 2020
I use this antibody as a secondary antibody for all western blots where primary antibody has been raised in mouse. Here I performed Western ...
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Rabbit Anti-Pax6 Antibody for Immunofluorescence
(1)
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BioLegend
October 09, 2020
Mouse embryonic Spinal cord sections E12.5 labeled by this Anti-Pax6 antibody.
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Detection of PKD1 in Primary Microglia
(1)
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Cell Signaling Technology
October 09, 2020
The antibody was used to detect changes in expression and/or localization of PKD1 in untreated and LPA treated primary microglia.
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Cleaved Caspase 3
(1)
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Cell Signaling Technology
October 09, 2020
I used this antibody to detect the apoptosis in THP-1 cells treated with different compounds.
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Caspase-8 Antibody for Western Blot
(1)
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Cell Signaling Technology
October 09, 2020
I used this antibody to detect total caspase 8 (as the loading control) in THP1 cells treated with different compounds.
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Detection of LPA Levels in Human Serum
(1)
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CUSABIO Technology LLC
October 08, 2020
The purpose of our experiment was to detect possible differences in the levels of LPA between control samples and samples from patients who ...
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iNOS Immunofluorescence in Primary Microglia
(1)
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Abcam
October 08, 2020
The antibody was used in order to detect iNOS expression in untreated and LPA treated microglia cells.
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Nice Caspase 3 Antibody
(1)
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Cell Signaling Technology
October 08, 2020
I used this antibody to probe for total caspase 3 (pro-apoptotic protein) in THP1 cells treated with different compounds.
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Nice Phospho Akt Antibody
(1)
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Cell Signaling Technology
October 07, 2020
I used this antibody to determine the basal levels of phospho Akt in different cancer cells.
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Determining the Inhibitory Synapses using Anti-Gephyrin Antibody
(1)
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Synaptic Systems GmbH
October 07, 2020
GFP transfected -hippocampal neurons were stained with anti-Gephyrin antibody after culturing them for 10 days in vitro (10DIV) to ...
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Great p27 Antibody
(1)
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Cell Signaling Technology
October 07, 2020
I used this antibody to determine check the efficiency of p27 knockdown in 293T cells. scramble siRNA and p27 specific siRNA-transfected ...
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Immunostaining with Sox2 Antibody
(1)
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R&D Systems, a Bio-Techne Brand
October 06, 2020
Adult mouse brain tissue was stained with SOX2 antibody to determine the neural stem cell population.
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Immunofluorescence Using PSD-95 Antibody in Cultured Hippocampal Neurons
(1)
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Abcam
October 06, 2020
GFP transfected -hippocampal neurons were stained with anti-PSD95 antibody after culturing them for 10 days in vitro (10DIV) to determine ...
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Detection of NADPH/NADP Ratio in BV2 Microglia Cells
(1)
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Abcam
October 06, 2020
We used the kit to quantify the levels of NADPH and NADP in BV2 microglia cells. Cells in the first experiment were treated with LPS (100ng/...
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Detection of IkBa Activation in BV2 Microglia Cells
(1)
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Cell Signaling Technology
October 06, 2020
In our experimental project we sought to determine downstream signaling pathways that are activated by lysophosphatidic acid (LPA). We ...
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Good Antibody for Clstn1
(1)
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R&D Systems, a Bio-Techne Brand
October 06, 2020
To detect the protein level of Clstn1. The cells were lysed and cell lysates were tested by WB.
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PTEN Antibody for Western Blot
(1)
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Cell Signaling Technology
October 06, 2020
I used this antibody to determine the levels of PTEN in 293T cells post transfection with siRNA against Akt.
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HPLC Binary Pump Works Well!
(1)
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Agilent Technologies
October 06, 2020
Worked really well!
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Must Have for Protein Crystallization Studies
(1)
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FORMULATRIX
October 05, 2020
The Rock Imager is an indispensable instrument for protein crystallization studies. You load the crystallization plate into the Rock Imager ...
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Detection of Total Nitric Oxide in Primary Microglia
(1)
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Enzo Life Sciences, Inc.
October 05, 2020
Primary murine microglia were treated with Lysophosphatidic acid (LPA) in the presence or absence of LPAR5 inhibitor. The kit was used to ...
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Detection of Total ROS Level in Primary Microglia
(1)
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Enzo Life Sciences, Inc.
October 05, 2020
Primary murine microglia cells were treated with LPA in the presence or absence of different MAPK inhibitors and the total ROS levels were ...
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Coomassie Fast and Easy to Use
(1)
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Carl Roth GmbH + Co. KG
October 05, 2020
ROTI®Blue is a colloidal Coomassie® brilliant blue G250 staining agent for proteins in polyacrylamid.
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Rat Anti-VEcadherin Antibody for Immunofluorescence
(1)
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MyBioSource.com
October 04, 2020
Decent antibody for labelling VE-cadherin. Optimization step might be needed.
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Detection of PKD2 in Primary Microglia
(1)
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Abcam
October 04, 2020
The antibody was used to detect changes in expression and/or localization of PKD2 in untreated and LPA treated primary microglia.
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GAPDH Antibody for Western Blot
(1)
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Cell Signaling Technology
October 04, 2020
GAPDH antibody was used to normalize western blot results in Hela cells.
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Detection of PLA2
(1)
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CUSABIO Technology LLC
October 04, 2020
The kit was used to detect the levels of PLA2 in serum samples from control patients and patients with neurodegenerative diseases.
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Crystal Violet Solution for Colony Formation Assays
(1)
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Merck
October 04, 2020
The solution was used to stain Hela cells colonies after 15 days of treatment with DMSO and inhibitory drug.
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Determining Cellular Cytotoxicity in Neurons
(1)
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Thermo Fisher Scientific
October 04, 2020
CATHa neurons were incubated for 24h with conditioned medium from control and treated microglia cells.
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Immunostaining for Frozen Sections
(1)
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Cell Signaling Technology
October 02, 2020
Yaptaz antibody was used in embryonic cortex to determine the presence of yaptaz in blood vessels and in the other regions of the tissue
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Immunostaining for Pioneer Axons Using TAG-1 Antibody
(1)
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Developmental Studies Hybridoma Bank / DSHB
October 02, 2020
TAG-1 antibody was used to label pioneer axons of the embryonic brain cortex.
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ß-Tubulin (9F3) Rabbit mAb (HRP Conjugate) for Western Blot
(1)
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Cell Signaling Technology
October 02, 2020
I used this antibody as a loading control for my samples stimulated with different compounds of interest.
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Immunostaining for Layer II/III marker in Brain Neocortex
(1)
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Abcam
October 01, 2020
CUTL1/CUX1 antibody was used to specifically label layer II/III neurons in the postnatal brain neocortex.
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Phospho-RIP (Ser166) Antibody for Western Blot
(1)
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Cell Signaling Technology
October 01, 2020
I used this antibody as an apoptotic marker in the untreated and stimulated THP-1 cells.
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Immunostaining for Axonal Fibers
(1)
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Developmental Studies Hybridoma Bank / DSHB
October 01, 2020
NFM-2H3 antibody was used to stain axonal fibres in the mouse embryonic brain cortex at E16
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Phospho-MLKL (Ser358) antibody for Western Blot
(1)
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Cell Signaling Technology
October 01, 2020
I used this antibody to detect the levels of apoptotic protein, phospho MLKL in THP-1 cells treated with different compounds.
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Neurofilament Immunofluorescence on Rat Sciatic Nerve Sections
(1)
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BioLegend
September 30, 2020
Used to replace Eurogentec SMI-35 antibody which has been discontinued. Clear staining with little background in rat sciatic nerve (15µm ...
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Great PI3 Kinase Antibody
(1)
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Cell Signaling Technology
September 30, 2020
I used this antibody to determine the endogenous levels of PI3 Kinase protein in human cancer cell lines. PI3 Kinase is one of the critical ...
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Great Pan Akt Antibody
(1)
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Cell Signaling Technology
September 30, 2020
I study the cancer cell proliferation pathways. Here, I was looking at endogenous levels of pan Akt in four cancer cells - 8505C, Cal62, ...
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Phospho-PI3 Kinase p85 Antibody Works Well
(1)
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Cell Signaling Technology
September 29, 2020
Mouse glioma CT2A cells were treated with Sorafenib for 3 hours and its effect on phospho PI3K levels was examined. Equal amount of ...
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Nice EGFR Antibody
(1)
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Cell Signaling Technology
September 29, 2020
EGFR is a receptor for epithelial growth factor that initiates cell proliferation, migration and invasion in cancer cells. I tested this ...
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Layer V/VI marker for Murine Brain- Immunostaining
(1)
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Abcam
September 29, 2020
The goal was to determine the layerV/VI positive neurons by immunostaining with Tbr1 in P12 postnatal brains.
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Immunostaining for Hif1 Alpha
(1)
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R&D Systems, a Bio-Techne Brand
September 29, 2020
Our goal was to determine the hypoxia levels by immunostaining with Hif1-alpha in embryonic brain sections.
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Great ELISA kit!
(1)
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RayBiotech
September 28, 2020
This kit gave me great results with LPS-inflammed RAW264.7 cells supernatants. The instructions were clear and detailed and it is made in a ...
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Great Mouse IL-6 ELISA Kit!
(1)
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RayBiotech
September 28, 2020
I used this kit with LPS-inflammed RAW264.7 cells supernatants. It was very sensitive! Each reagent was labeled clearly and the instructions...
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Anti-FOXM1 Worked Well for Us
(1)
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Abcam
September 27, 2020
I used this antibody to the levels of FOXM1 in mouse (C57BL6) spleen ,thymus and lymph nodes.
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Goat Anti-Human IgG(H+L) antibody for ELISA
(1)
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SouthernBiotech
September 27, 2020
It's a secondary antibody conjugated with HRP. We used this antibody for detecting specific human IgGs using the ELISA method. It's a ...
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