Immunostaining for Neuronal Nuclei

November 18, 2020

BlueskyReddit
Vascular Dysfunction
University of Heidelberg
Postdoctoral Researcher

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Company:

Millipore

Product Name:

NeuN anitbody

Catalog Number:

MAB377

Clone Number:

A60

NeuN antibody was used to label the neurons and determine their layer position in the cortex at P5

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

vibratome sectioned mouse brain tissue at P5

Primary Incubation

1:200, 4 degrees overnight

Blocking Agent

10% Normal donkey serum in PBST (0.5% Triton X 100)

Secondary Incubation

1:500, AF 488

Tertiary Incubation

None

Detection

Immunofluorescence

Results Summary

Neuronal nuclei were specifically labelled in the whole P5 brain -wherever neurons were present.

DOI or PMID #

N/A

Additional Notes

Antigen retrieval in 10mM Sodium citrate buffer (pH 6) may improve staining, but it works without that also

Image Gallery

Summary

The Good

Specific staining, good for thin sections

The Bad

None

The Bottom Line

Antibody has a great signal, highly recommended.

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