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Date Published
Detection of Phospho AKT in Primary Microglia
(1)
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Cell Signaling Technology
October 30, 2020
Primary murine microglia were treated with LPA in the presence or absence of LPAR5 or PKD family inhibitors in order to detect changes in ...
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Protein Estimation Using Pierce BCA Protein Assay Kit
(1)
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Thermo Fisher Scientific
October 30, 2020
We measured the protein content of the cell lysate using this kit to normalise our results. 10ul of the sample was mixed with 200ul of the...
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Neoteryx 10 ul Mitra Cartridge (2-Sampler) Works Great!
(1)
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October 30, 2020
Perfect for the remote times in which we are all now conducting research studies!
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P-MYPT1 for Western Blot
(1)
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Cell Signaling Technology
October 30, 2020
The antibody signal very good.
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CXCR5 Rat anti-Human, Alexa Flour 647 Works Well for Flow Cytometry!
(1)
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October 30, 2020
We are trying to study the T follicular regulatory cells, which is CD4+CXCR5+Foxp3+, we'd like to know how is the percentage difference ...
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An Efficient Elisa Kit for IL-6 Quantification
(1)
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Proteintech Group Inc
October 29, 2020
We used the kit in order to quantify the levels of IL-6 in the supernatants from untreated and LPA treated BV2 and primary microglia cells.
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P-AMPK for Western Blot
(1)
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Cell Signaling Technology
October 28, 2020
When heavy bleeding occurs, there’s not enough blood flow to the organs in your body. Blood carries oxygen and other essential substances to...
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Phospho Erk1/2 for Western Blot
(1)
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Cell Signaling Technology
October 28, 2020
This antibody produced too many nonspecific bands to be useful.
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P-AKT Western Blot from CST
(1)
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Cell Signaling Technology
October 28, 2020
Oxygen is a vital element and toxicity may not be immediately obvious. Moreover, impaired oxygen delivery in critically ill patients is ...
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Anti-phosph p38 MAPK for Western Blot
(1)
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Cell Signaling Technology
October 28, 2020
The antibody 9211 against designed to identify the phosphorylated p#8 MAPK. However, the antibody bound to too many nonspecific bands in the...
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Phospho-SHIP1 (tyr1020) antibody
(1)
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October 27, 2020
Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with ...
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NLRP3 Works Great for Western Blot
(1)
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Cell Signaling Technology
October 27, 2020
NLRP3 (NOD-, LRR- and pyrin domain-containing protein 3) is an intracellular sensor that detects a broad range of microbial motifs, ...
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PE anti-human CD62L Antibody Works Great!
(1)
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BioLegend
October 27, 2020
We use it to gate CD62L+ cells, trying to study the different percentages of CD62L+ between healthy and patient PBMCs.
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Works Well for Flow Cytometry!
(1)
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Thermo Fisher Scientific
October 27, 2020
Use it to tell CD4 Naive cells, which is CD4+and CD45RA+ in both healthy and patient PBMCs, to compare the percentage between them.
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KCNMB4 Works Great
(1)
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Thermo Fisher Scientific
October 26, 2020
MaxiK channels are one of the large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of ...
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Cleaved Caspase-8 Antibody Works Well
(1)
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Cell Signaling Technology
October 26, 2020
I used this antibody to detect apoptosis in THP1 cells treated with different compounds.
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COX IV (3E11) Rabbit mAb (HRP Conjugate) for Western Blot
(1)
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Cell Signaling Technology
October 26, 2020
THP-1 cells treated with different stimulants were used to isolate the mitochondrial fraction.
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LRRC26 for Western Blot
(1)
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Alomone Labs, Ltd.
October 26, 2020
Leucine-rich repeat containing protein 26 (otherwise called as BK channel auxiliary gamma subunit or LRRC26) is a 37 kDa single spanning ...
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Biotin Anti-Mouse CD127 (IL-7Ra) Antibody
(1)
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BioLegend
October 26, 2020
Immunoprecipitation with Biotin anti-mouse CD127 (IL-7Ra) Antibody
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Immunoprecipitation with Biotin Anti-Mouse CD19 Antibody
(1)
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BioLegend
October 23, 2020
Immunoprecipitation with Biotin anti-mouse CD19 Antibody of protein from mice splenic B cells
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PARP Antibody from CST
(1)
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Cell Signaling Technology
October 23, 2020
PARP antibody has been used to detect the activation of apoptosis related pahtways followinf treatment with two compounds.
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CellTiter Blue 20 mL Promega
(1)
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Promega
October 22, 2020
HelaS3 cell viability has been measured via CellTiter blue assay after treatment with increasing drug concentrations.
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Great Western Blot Antibody
(1)
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Abcam
October 22, 2020
I use this antibody for Western blotting while probing primary antibody is raised in rabbit.Here, I probed Beta actin in THP1 cells using ...
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Easy F-actin Detection
(1)
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Thermo Fisher Scientific
October 22, 2020
Using Thermo Scientific's Alexa Fluor 488 Phallodin makes F-actin detection a breeze. Useful for many types of imaging to mark the cell ...
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Goat Anti-Mouse IgG H&L for Western Blot
(1)
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Abcam
October 22, 2020
I use this antibody as the secondary antibody for Western blotting to detect primary antibodies raised in mouse. Here, I am showing the beta...
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Matrix for MALDI TOF Analysis
(1)
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Thermo Fisher Scientific
October 22, 2020
We conduct MALDI experiment for MS peptide analysis. It is now easier to perform with single-use, highly pure MALDI matrices that are ...
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Efficient Ultra-Centrifuge
(1)
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October 22, 2020
We use this ultra-centrifuge for separating unentrapped drug from drug liposome liposomes. Additionally, it is also capable of separating ...
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P21 from CST, A Good Antibody
(1)
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Cell Signaling Technology
October 22, 2020
Staining of p21 in human Hela S3 cells, good signal overall.
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CD45RO Antibody Works Well for Flow Cytometry
(1)
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BioLegend
October 21, 2020
We studied human T helper cell responses, with a focus on different subsets such as Tfh cells that ensure potent antibody responses. Immune ...
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Extracellular GLUT1 Antibody for Flow Cytometry
(1)
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Alomone Labs, Ltd.
October 21, 2020
BV2 microglial cells were treated with LPS, and then detected for extracellular GLUT1 by this antibody by flowcytometry.
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Thermo Scientific™ GeneRuler 1 kb DNA ready to use 250 – 10,000 bp
(1)
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Thermo Fisher Scientific
October 21, 2020
Sizing of double-stranded DNA on agarose gels.
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Isotype Control for Flow Cytometry
(1)
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Thermo Fisher Scientific
October 21, 2020
BV2 microglia cells were treated with LPS and stained with isotype control and compared with unstained and GLUT1-FITC stained cells
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Good Benchtop Incubator Shaker
(1)
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Eppendorf
October 21, 2020
We incubate and shake our bacterial culture flask for our molecular biology based experiments.
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Good Microcentrifuge
(1)
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Eppendorf
October 21, 2020
We often centrifuge our samples at different temperature and speed. It works really well for small volume samples too.
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Diacylglycerol (DAG) Antibody -FACS
(1)
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Abbexa Ltd
October 20, 2020
Staining of B cells from mice bone marrow after cytoplasmic permezbiliation with Diacylglycerol (DAG) Antibody from abbexa
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Phospho-CD79A (Tyr182) Antibody for Flow Cytometry -CD79A (Tyr182) Antibody
(1)
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Cell Signaling Technology
October 20, 2020
Staining of B cells from mice bone marrow after cytoplasmic permezbiliation with Phospho-CD79A (Tyr182) Antibody from Bioké
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Caspase-3 Antibody from (CST)
(1)
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Cell Signaling Technology
October 19, 2020
Caspase-3 has been used against the WCL of Hela S3
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IL1-beta for Western Blot
(1)
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Cell Signaling Technology
October 16, 2020
Hyperoxia involves the administration of high concentrations of inspired oxygen to the lung. Hyperoxemia reflects high levels of oxygen in ...
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P-PKC Alpha and Beta II for Western Blot
(1)
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Cell Signaling Technology
October 16, 2020
Hyperoxia leads to development of reactive oxygen species (ROS). These ROS cause oxidative damage to the lung, nucleic acids, and proteins, ...
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P-MAPK for Western Blot
(1)
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Cell Signaling Technology
October 15, 2020
Trx system is responsible for protecting cells from oxidative stress, and is composed of several related molecules that form a network of ...
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MFAP5 Expression in Skin Fibroblasts
(1)
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Thermo Fisher Scientific
October 14, 2020
The aim was to detect MFAP5 on fibroblasts using flow cytometry. This antibody works great.
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Analyzing BKBeta4 Expression
(1)
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OriGene Technologies
October 14, 2020
MaxiK channels is a large conductance, voltage and calcium-sensitive potassium channels and fundamental for the control of smooth muscle ...
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Very Good Anti-Tubulin Antibody
(1)
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Santa Cruz Biotechnology, Inc.
October 14, 2020
Protein expression in Hela S3 has been tested and normalized against the tubulin. The signal obtained after incubation with the following ...
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CD49b on Fibroblasts
(1)
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Thermo Fisher Scientific
October 14, 2020
The aim was to detect CD49b on human dermal fibroblasts using flow cytometry. The antibody worked well.
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P53 Antibody HEK293 Cells
(1)
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Cell Signaling Technology
October 14, 2020
The P53 antibody has been used in HEK293 cells do detect the endogenous P53. Despite the strong signal obatined, the antibody needs some ...
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TGF-alpha in Mouse Skin Wounds
(1)
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MyBioSource.com
October 13, 2020
The aim was to identify cell producing TGF-a in mouse skin wounds. The antibody works well.
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Measuring -KCNQ4 expression
(1)
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OriGene Technologies
October 13, 2020
The protein encoded by this gene forms a potassium channel and its play a vital role in the regulation of neuronal excitability, ...
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c-Myc antibody (D84C12) Rabbit mAB
(1)
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Cell Signaling Technology
October 13, 2020
Good antibody for the detection of c-Myc and very sensitive to c-Myc expressions changes
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Anti-Histone H2A.X Mouse mAb
(1)
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Cell Signaling Technology
October 13, 2020
The antibody was used to detect the phosphorilation of the Ser139 following the addition of a chemical compound, thus evaluating DNA damage ...
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MFAP5 in Mouse Skin Wounds
(1)
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MyBioSource.com
October 13, 2020
The aim was to find out the expression patterns of MFAP5 in mouse skin wounds. The antibody works great to stain MFAP5 in both epidermis ...
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