Immunostaining for Axonal Fibers

November 18, 2020

BlueskyReddit
Vascular Dysfunction
University of Heidelberg
Postdoctoral Researcher

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Company:

MIllipore

Product Name:

Neuronal Cell Adhesion molecule L1

Catalog Number:

MAB5272

Clone Number:

324

Info: Supplier Page 

Browse Similar Products: L1 Immunofluorescence Antibodies

L1 was used for staining the cortical axons-using it as a pan axonal marker.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Frozen embryonic mouse brain tissue (E13)

Primary Incubation

1:200, 4 degrees overnight

Blocking Agent

10% Normal donkey serum in PBST (0.5% Triton X 100)

Secondary Incubation

1:500, AF 488

Tertiary Incubation

None

Detection

Immunofluorescence

Results Summary

L1 specifically marked the cortical axons in E13 brain cortex.

DOI or PMID #

N/A

Additional Notes

Antigen retrieval should NOT be performed

Image Gallery

Summary

The Good

Specific staining, good for thin sections

The Bad

None

The Bottom Line

Good signal, recommended-would buy again

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