Relatively Dim Antibody for Granzyme B

November 25, 2020

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Pacific Blue™ anti-human/mouse Granzyme B Antibody

Catalog Number:

515408

Clone Number:

N/A

Since our lab was interested in looking at T cells, specifically CD8 and CD4, this molecule was of interest due to its expression levels upon activation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine T Cells

Primary Incubation

1:500 in BD Perm Wash

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow BD Celesta

Results Summary

It may be relatively dim at times due to the nature of the fluorescence but it is useful for when we are trying to identify the population with high Granzyme B in CD8 Tcells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

It is relatively great for gating out positive and negative signal

The Bad

You might need to titrate it well depending on your cell of interest

The Bottom Line

This is a pretty good antibody just to gate out the negative and positive signals.

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