Cell Signaling
Phospho-p38 MAPK (Thr180/Tyr182) Antibody #9211
9211
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Primary murine microglia were treated with LPA in the presence or absence of LPAR5 (TCLPA5) or PKD family (CRT) inhibitors in order to detect changes in the phosphorylation of MAPK family members.
Western Blot
primary murine microglia
1:1000 overnight at +4
5% milk
1:5000 goat anti-rabbit, 2 hours at room temperature
N/A
ChemiDoc System
Treatment with LPA increased the expression of pp38 in microglia cells. The expression appeared to be both LPAR5 and PKD dependent.
Depending on starting material, a dilution 1:500 may work better.
Reliable with a very good signal
A "standard" for the detection of phospho p38!