Memory Marker on CD8

Oncology
Johns Hopkins School of Medicine
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

CD122 Monoclonal Antibody in FITC

Catalog Number:

11-1222-82

Clone Number:

N/A

CD122 is normally used as a memory marker for our CD8 T cells. Our lab is interested in looking at the persistence of memory cell post stimulation both in-vitro and in-vivo. In our case we had used it on cells that were activated and then treated with Rapamycin.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine T cells

Primary Incubation

1:400 in FACS buffer

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Celesta

Results Summary

This works very well to gate out the CD122+ and CD122- cell population, thus being able to look at CD8s memory cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Antibody works well every time

The Bad

Price is pretty high

The Bottom Line

This antibody works well and should be included in other people's panel creation.

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