Good Antibody to Study TSC2 Activity (S939)

November 24, 2020

Immunobiology
Yale University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies (CST)

Product Name:

Phospho-Tuberin/TSC2 (Ser939) Antibody

Catalog Number:

3615

Clone Number:

N/A

TSC2 as a negative regulator of mTORC1 activity is phosphorylated at S939 to become inactivated. This antibody recognizes this phosphorylated state.

Experimental Design and Results Summary

Applications

Western Blot

Sample

T cells

Primary Incubation

1:2000 in 4% BSA/TBST

Blocking Agent

5 percent milk in TBST

Secondary Incubation

1:5000 anti-Rabbit HRP in 5% milk/TBST for 1hour

Tertiary Incubation

None

Detection

Digital imager using ECL

Results Summary

We are able to see increase in phosphorylation with stimulation compared to untreated.

DOI or PMID #

None

Additional Notes

Have a proper control to see baseline and then increase.

Image Gallery

Summary

The Good

Can detect right band and is generally reliable even though polyclonal.

The Bad

Polyclonal so a bit more messier.

The Bottom Line

Recommend to purchase to look at this important site on TSC2.

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