Great Multi-Purpose Antibody for TSC2

Immunobiology
Yale University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies (CST)

Product Name:

Tuberin/TSC2 XP Rabbit mAb

Catalog Number:

4308

Clone Number:

D93F12

TSC2 is a negative regulator of mTORC1. We use this antibody to check for TSC2 in our cells by western.

Experimental Design and Results Summary

Applications

Western Blot

Sample

T cells

Primary Incubation

1:2000 in 4% BSA/TBST

Blocking Agent

5 percent milk in TBST

Secondary Incubation

1:5000 anti-Rabbit HRP in 5% milk/TBST for 1hour

Tertiary Incubation

None

Detection

Digital imager using ECL

Results Summary

We are able to detect robust signal at the appropriate size band in our western.

DOI or PMID #

N/A

Additional Notes

Titrate antibody based on samples

Related Categories

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Summary

The Good

Great reliable antibody with minimal noise.

The Bad

None

The Bottom Line

Highly recommend for western blot analysis.

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