Great Antibody to Detect CD71 Expression

November 23, 2020

Immunobiology
Yale University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE anti-mouse CD71 Antibody

Catalog Number:

113808

Clone Number:

RI7217

CD71 is the transferrin receptor found on T cells. It is increased upon T cell activation therefore we look at it to assess mTOR dependent processes.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

T cells

Primary Incubation

1:2000

Blocking Agent

FC Block in FACS buffer during surface staining

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD Celesta

Results Summary

We are able to see huge increases in CD71 expression on T cells upon activation.

DOI or PMID #

None

Additional Notes

Titrate antibody based on samples

Related Categories

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Summary

The Good

Great robust antibody.

The Bad

None

The Bottom Line

Highly recommend to anyone looking at CD71 by flow.

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