Great Flow Antibody to Detect LAG3 Expression on T Cells

Immunobiology
Yale University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-mouse CD223 (LAG-3) Antibody-Brilliant Violet 785

Catalog Number:

125219

Clone Number:

C9B7W

To detect T cell exhaustion, LAG3 is an important marker to identify exhausted T cells with PD1 expression. This antibody detects LAG3 expression using flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Tumor lymphocytes

Primary Incubation

1:400

Blocking Agent

FC Block in FACS buffer during surface staining

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytek Aurora (3 laser, BVR)

Results Summary

Really identifies dysfunctional exhausted CD8+ T cells using flow cytometry.

DOI or PMID #

NA

Additional Notes

Titrate antibody based on tissue samples

Related Categories

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Summary

The Good

Robustly identifies exhausted T cells in the tumor.

The Bad

None

The Bottom Line

Great antibody to detect LAG3+ T cells in the tumor.

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