Awesome Antibody to Detect KLRG1 Expression on T Cells

November 20, 2020

Immunobiology
Yale University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

ThermoFisher eBioscience

Product Name:

KLRG1 Monoclonal Antibody-PE-eFluor 610

Catalog Number:

61-5893-82

Clone Number:

2F1

KLRG1 is a terminal effector marker on CD8+ T cells to identify differentiated T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine splenocytes

Primary Incubation

1:1000

Blocking Agent

FC Block in FACS buffer during surface staining

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD Celesta

Results Summary

We can identify KLRG1 expression on CD8+ T cells.

DOI or PMID #

NA

Additional Notes

Titrate antibody based on tissue samples

Related Categories

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Summary

The Good

Robustly identifies KLRG1+ T cells in the tumor.

The Bad

None

The Bottom Line

Awesome antibody to detect KLRG1 at a nice dilution to save money.

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