Nice Antibody to Detect CD90.2 T Cells

Immunobiology
Yale University
Postdoctoral Researcher

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Company:

Biolegend

Product Name:

Anti-mouse CD90.2 Antibody-Brilliant Violet 510

Catalog Number:

105335

Clone Number:

30-H12

CD90.2 is a congenic marker expressed on T cells. It's typically used to identify donor T cells in adoptive transfer experiments.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine splenocytes

Primary Incubation

1:500

Blocking Agent

FC Block in FACS buffer during surface staining

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD Celesta

Results Summary

We can identify CD90.2 CD8+ T cells in the spleen from donors and host.

DOI or PMID #

NA

Additional Notes

Titrate antibody based on tissue samples

Related Categories

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Summary

The Good

Robustly identifies CD90.2 donor CD8 T cells in the spleen.

The Bad

None

The Bottom Line

Nice bright antibody to detect donor T cells based on congenic marker CD90.2.

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