Good CD95 Monoclonal Antibody from Miltenyi

November 19, 2020

Lehrstuhl Neurologie
University Regensburg
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD95 (FAS) Antibody, anti-human, FITC

Catalog Number:

130-124-214

Clone Number:

N/A

Here, we analyzed the presence of CD95 positive cells in mycosis fungoides by using the MACSima multi staining system.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human skin (lymphoma)

Primary Incubation

1:10 for 1 h at room temperature

Blocking Agent

3% BSA in PBS

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence microscopy

Results Summary

I was able to detect CD95 expression in the analyzed tissue.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use, specific with low background

The Bad

Not very bright

The Bottom Line

Inexpensive antibody and easy protocol -> good choice.

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