Antibody Works Well

Medicine
Harvard Medical School
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

IBT Bioservices

Product Name:

rabbit anti-LukF-PV antibody

Catalog Number:

0312-002

Clone Number:

N/A

Info: Supplier Page

Browse Similar Products: anti-LukF Western Blot  Antibodies

I am working on the pathogenesis of Staphylococcus aureus infections. I am interested in the role of staphylococcal toxins in bacterial pathogenesis. I use this antibody to detect the LukF-PV leukocidin

Experimental Design and Results Summary

Applications

Western Blot

Sample

purified LukF-PV toxin

Primary Incubation

1 ug/ml concentration for overnight at 4 degree

Blocking Agent

5% skim milk

Secondary Incubation

1:10000 dilution and incubate 1 h at room temperature

Tertiary Incubation

N/A

Detection

chemilluminance

Results Summary

We loaded 80 ng of purified LukF-PV toxin on SDS-PAGE gel and the protein was transferred to PVDF membrane. The protein on the membrane was probed by anti-LukF-PV antibody and we got a nice signal. We recommend it

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very sensitive and cheap

The Bad

No

The Bottom Line

Generally it is a very good antibody and it can specifically detect the F subunit of staphylococcal leukocidin PVL.I highly recommend it.

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