Expression of LPAR2 in Microglia Cells

November 24, 2020

BlueskyReddit
Molecular Biology and Biochemistry
Medical University of Graz
Postdoctoral Researcher

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Company:

Origene

Product Name:

EDG4 (LPAR2) Rabbit Polyclonal Antibody

Catalog Number:

AP05151PU-N

Clone Number:

N/A

BV2 microglia were treated with LPS or IL4 and the expression of different LPA receptors was detected using immunoblotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

BV2 microglia cells

Primary Incubation

1:400 overnight at +4

Blocking Agent

5% milk

Secondary Incubation

1:5000 goat anti-rabbit, 2 hours at room temperature

Tertiary Incubation

n/a

Detection

ChemiDoc System

Results Summary

The expression of LPAR2 was increased only after LPS treatment.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very good signal

The Bad

N/A

The Bottom Line

A good antibody for LPAR2 detection.

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