BD Biosciences
BV605 Rat Anti-Mouse CD5
563194
53-7.3
Info:Supplier Page
Browse Similar Products: CD5 Antibodies
Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of B cells and antibody-secreting cells (ASC) in health and disease, in this experiment we are looking to try an anti-CD5 in T cells to make sure it works.
Flow Cytometry
Mouse spleen
Surface staining with CD5 BV605 (1/100), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer with a violet laser
We gated lymphocytes, singlets and live cells (PI-). Then, in the last dot plot it can be observed that CD3+ T cells express CD5, as expected. The T cells are CD5+.
Reliable
Weak signal and expensive.
It works, but there are better options in the market.