Effective Lentiviral Particle for Mito-GFP Expression

Houston methodist hospital
Postdoctoral Researcher

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Company:

Takara Bioscience

Product Name:

rLV.EF1.AcGFP1-Mito-9

Catalog Number:

0017VCT

Purchased lentiviral vector that containing a specific mitochondria-targeting sequence for the purpose of labeling mitochondria in primary mesenchymal stem cells. The vector was transduced into the cells at a multiplicity of infection (MOI) of 5, resulting in successful expression of green fluorescent protein (GFP) specifically in the mitochondria. This confirms the specificity and efficiency of the vector for labeling mitochondria in primary mesenchymal stem cells.

Experimental Design and Results Summary

Application

Mitochondria labeling

Starting Material

Mesenchymal stem cell

Tips

Correct titration of viral vector.

Results Summary

Data is showing highly efficient expression of GFP in the specific cellular organelle, mitochondria.

DOI or PMID #

N/A

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Summary

The Good

Visualization of mitochondria.

The Bad

Contains viral particles. May expect safety issues.

The Bottom Line

This is quite expensive. But highly recommend to create a specific cell line contains the mitochondria specific-GFP expression site, which may help your mitochondria related work.

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