Easy to Use and Reliable Method to Isolate Monocytes from PBMC

March 09, 2023

BlueskyReddit
MD Anderson Cancer Center - University of Texas
Cancer System Imaging (Diagnostic Imaging Division)
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Pan Monocyte Isolation Kit, human

Catalog Number:

130-096-537

In that experimental context, the Pan Monocyte Isolation Kit is used as a labeling system for the isolation of untouched monocytes from human PBMCs. The cells are then used in our in-vitro experiments to study inflammatory conditions related to the immunity system with a luminol-based probe to image reactive (oxygen and nitrogen) species. We chose the product for its easy to use but overall it always gave us reliable results.

Experimental Design and Results Summary

Application

Isolation of untouched monocytes from human PBMCs

Starting Material

Human PBMCs

Tips

Follow the protocol concentration to have the best results.

Results Summary

In that experimental context, the Pan Monocyte Isolation Kit is used as a labeling system for the isolation of untouched monocytes from human PBMCs. The cells are then used in our in-vitro experiments to study inflammatory conditions related to the immunity system with a luminol-based probe to image reactive (oxygen and nitrogen) species. Overall it always gave us reliable results.

DOI or PMID #

N/A

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Summary

The Good

Easy to use, reliable results.

The Bad

N/A

The Bottom Line

Easy to use and reliable method to isolate monocytes from PBMC. In that experimental context, the Pan Monocyte Isolation Kit is used as a labeling system for the isolation of untouched monocytes from human PBMCs. The cells are then used in our in-vitro experiments to study inflammatory conditions related to the immunity system with a luminol-based probe to image reactive (oxygen and nitrogen) species. We chose the product for its easy to use but overall it always gave us reliable results.

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