Thermo Fisher Scientific (Invitrogen)
CD170 (Siglec F) Monoclonal Antibody (1RNM44N), NovaFluor™ Blue 610-70S, eBioscience
M042T03B06
N/A
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We are interested in studying the mechanisms responsible for ensuring T cell self tolerance that rely on thyme and extra-thymic Aire expressing cells.
Flow Cytometry
Mouse thymus
Live dead: Zombie NIR LD 1:4000 in 100ul FACS for 20 min at RT
2.4G2
Surface stains: SiglecF 1:800 in 100uL FACS for 30 min at 4C
PFA (15 min RT) + FoxP3 Fix/Perm kit (30 min 4C), then intracellular stain in 100uL perm buffer for 1hr at RT
Cytek Aurora spectral flow cytometry
This antibody gives good, strong staining and separation for neutrophils from other thymic cell populations. This allows us to remove neutrophils from downstream analysis because they tend to be highly auto-fluorescent by spectral FC.
Good staining for neutrophils.
None
Useful antibody for removing auto fluorescent cells from analysis.