Great Iba1 Antibody for Immunofluorescence

March 16, 2023

Anatomy and Cell Biology
University of Illinois at Chicago
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

Recombinant Anti-Iba1 antibody

Catalog Number:

ab178847

Clone Number:

EPR16589

Info:Supplier Page View Product Specs

Browse Similar Products: Iba1 Antibodies

Neuroinflammation and microglial activation as a result of respiratory infection was analyzed by immunofluorescence.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain section

Primary Incubation

1:100 in PBS+0.1% Triton-X100 overnight incubation at 4C

Blocking Agent

PBS+0.1% Triton-X100 + 10% BSA

Secondary Incubation

Rat anti-rabbit IgG AF488 1:500 in PBS+0.1% Tween-20 2hr at 25C

Tertiary Incubation

N/A

Detection

Epifluorescence microscopy

Results Summary

Enhanced Iba1 immunofluorescence signal in hippocampal microglia was detected in mice infected with respiratory virus.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good signal, works with mouse, rat, and human samples.

The Bad

Expensive antibody.

The Bottom Line

This antibody is expensive but works very well for immunofluorescence. Would highly recommend.

Share your experience with other scientists. Write a Review! »

Join the discussion