Novus Biologicals, LLC
58K Golgi Protein Antibody (58K-9)
NB600-412SS
58K-9
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In the context of observed apoptosis, 58K Golgi protein was selected to assess fragmentation of Golgi apparatus in isolated and cultured mouse brain endothelial cells (mBECs). The cultured mBECs were treated with a pro-inflammatory cytokine for 36 hours. The cells were then fixed and stained to assess morphological state of the Golgi using unpurified Mouse 58K Golgi Protein Monoclonal Antibody (Catalog # NB600-412SS) at a dilution of 1:100 overnight at 4 °C. DAPI was used as a nuclear stain with Ibidi Mounting Media. Additionally, we stained for ICAM-1, intracellular adhesion molecule 1.58K in green, DAPI in blue, ICAM-1 in greyThis image was taken on a Zeiss LSM 710 Confocal Microscope (META) with Zeiss Plan Apo 63x/1.40 oil
Immunohistochemistry
Mouse, brain endothelial cells
1:100, Over-night, 4°C
10% BSA/PBST
Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)
N/A
Confocal Microscopy
Fragmentation of the Golgi apparatus in the context of observed apoptotic mouse brain endothelial cells in-vitro following prolonged exposure to pro-inflammatory cytokine. Note chromatin condensation and partial DNA fragmentation as observed by DAPI stain.
Accessible and good signal.
Effective and affordable. We will continue to use this antibody as a Golgi marker.