Abcam
Recombinant Anti-CD39 antibody [EPR3678(2)] (ab108248)
ab108248
N/A
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We have been using this antibody in our investigations of ectonucleotidases in the context of viral lymphomas. Here we used CRISPR to generate a CD39-KO lymphoblastoid cell line and used this antibody to confirm the knockout.
Western Blot
Human lymphoblastoid cell lines
1:1000 in milk, incubated at 4C overnight
Milk
1:5000 in milk, incubated at room temp for one hour
Chemiluminescence
We have been using this antibody to consistently detect CD39 protein expression in various human B cell lines. Here we used the antibody to confirm the lack of CD39 protein expression in a CRISPR-generated knockout.
Reliably detects endogenous CD39.
A little pricey.
Been using this antibody for years and, while pricey, it has worked well consistently.