Great Transfer Apparatus

August 14, 2014

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Jai Narain Vyas University, Jodhpur
Department of Biology
PhD student

Company:

Bio-Rad

Product Name:

Mini Trans-Blot Module

Catalog Number:

170-3935

I use this apparatus to transfer proteins from SDS-PAGE gels to PVDF membranes for immunoblotting.

We work on DNA damage pathways in our lab. This involves running a lot of gels for immunoblotting. The Biorad transfer apparatus is easy to use and one gets a very uniform and effecient transfer of proteins to PVDF membranes. That is why I chose to use this apparatus.

Experimental Design and Results Summary

Tips

Check for the right orientation (black and red) and bubbles between the gel and membrane

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Summary

The Good

Can be assembles easily. Possible to use for one or two gels

The Bad

Nothing to mention

The Bottom Line

I will always use this apparatus from BioRad for all my immunoblot transfers.

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