SF2/ASF protein expression analyzes via Western blot

October 14, 2014

Kli.Pha.-Tox
Charité - Universitatsmedizin Berlin, CCM
Ph.D.

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Identification of SF2/ASF protein using SF2/ASF monoclonal mouse antibody via western blot.

Company:

Life Technologies

Product Name:

SF2/ASF Monoclonal Antibody, Mouse (96)

Catalog Number:

324500

Info: View Product View Product Specs

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SF2/ASF protein expression was characterized via western blot using the SF2/ASF Antibody (dilution 1:1000).

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysate of human endothelial cells

Primary Incubation

1:1000; over night , 4°C , in TBS-T; 1%BSA; 0.02 Na3

Blocking Agent

3% BSA solution

Secondary Incubation

1:5000, 1 h, bei room temperature, goat-anti-rabbit IgG, HRP-conjugated

Tertiary Incubation

5% low-fat milk solution.

Detection

ECL Plus Detection Reagents (Amersham)

Results Summary

Using the above mentioned antibody (1:1000) one band was detected for SF2/ASF at approximately 30 kDa.

Additional Notes

NA

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Identification of SF2/ASF protein using SF2/ASF monoclonal mouse antibody via western blot.

Summary

The Good

SF2/ASF was detected by this antibody in a clear band

The Bad

NA

The Bottom Line

This antibody works well for Western blotting.

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