Promega
Caspase-Glo® 3/7
G8091
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An easy to way to measure cell death in a plate-based assay. This kit allows the scientist to rapidly measure the levels of caspase-3/7 activation in vitro. This assay complements other commonly used assays for cell death such as Annexin V/PI staining.
Cell death assay - luciferase based assay (96-well plate option)
Adherent cells and a 96-well dish
MEFs were seeded in 96-well plates and stimulated. The luciferase-based caspase assay was used to measure Caspases-3/7 activity following stimulation according to manufacturer’s protocol. Samples were read on a multilabel automated plate reader (Perkin Elmer) and triplicates were performed for each condition (means ± SD are presented).
Reduce the volume by 50% to extend the number of assays that can be performed.
WT (black) and KO (white) MEFS were stimulated as shown and caspase-3/7 activity was measured. Results are presented as ''fold activation'' compared to the control for each genotype (ST, Staurosporine).
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Rapid assay that gives consistent reults.
Can be expensive.
Overall great assay that efficiently measures caspase-3/7 activity in cultured cells.
Product Name: Caspase-Glo 3/7 AssaySupplier Page