Sigma
Monoclonal anti-FLAG M1 antibody produced in mouse
F3040
Browse Similar Products: FLAG Western blot Antibodies
We used this antibody for Western blotting of FLAG-tagged proteins expressed in mammalian cell lines. Works well if stored properly. Regular freezing and thawing results in poor results.
Western Blot
Mammalian cell lysate
1:2500, Milk, Room Temperature, 1.5 hours
Milk
1:10000, Milk, Room Temperature, 1 hour
NA
ECL plus
Very good bands at 10 ug total input protein
Specificity
Stability upon multiple freezings and thawings.
Good for Western blot.