FGF2 protein expression via western blotting

October 27, 2014

Kli.Pha.-Tox
Charité - Universitatsmedizin Berlin, CCM
Ph.D.

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FGF2 protein identified using anti-FGF-2/bFGF antibody via Western blot.

Company:

Merck Millipore

Product Name:

Anti-FGF-2/bFGF

Catalog Number:

MABS72

Info: View Product View Product Specs

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FGF2 protein expression was characterized via western blot using the Anti-FGF-2/bFGF Antibody (1:500) in murine HL-1 cells.

Experimental Design and Results Summary

Applications

Western blot

Sample

Lysate of murine HL-1 cells

Primary Incubation

1:500; overnight , 4°C , in TBS-T; 1%BSA; 0.02 Na3

Blocking Agent

5% low-fat milk solution

Secondary Incubation

1:5000, 1 h, room temperature, rabbit-anti-mouse IgG – HRP conjugated

Tertiary Incubation

5% low-fat milk solution

Detection

ECL Plus Detection Reagents (Amersham)

Results Summary

Using the above mentioned antibody (1:500), several bands were detected including a FBF2 band at approximately 17 kDa.

Additional Notes

NA

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FGF2 protein identified using anti-FGF-2/bFGF antibody via Western blot.

Summary

The Good

FGF2 was detected by this antibody (17kDa).

The Bad

Some other bands were detected by this antibody in higher ranges.

The Bottom Line

This antibody is sufficient for protein expression analyses via Western blotting.

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