Bethyl Laboratories
PanK4 Polyclonal Antibody,
A303-776A
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Antibody successfully IP'd PANK4 from MCF10a cells, but quite a messy blot and needs a lot of starting material.
Immunoprecipitation
MCF10a cells
1:50 overnight at 4 degrees
5% BSA
1:1000 in 5% BSA for 1 hour at room temperature
N/A
ECL reagent
Good antibody for IP'ing PANK4 from mammalian MCF10a cells, but needs a lot of starting material. Good signal by Western blotting.
Easy to use.
Needs a starting material.
Would use it again.