Anti-Mouse CD90.2 Flow Cytometry Antibody From BD Biosciences

September 18, 2023

BlueskyReddit
NOMIS CENTER FOR IMMUNOBIOLOGY AND MICROBIAL PATHOGENESIS
The Salk Institute of Biological Studies
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BD OptiBuild™ BUV563 Rat Anti-Mouse CD90.2

Catalog Number:

741213

Clone Number:

53-2.1

I study tumor immune microenvironment in preclinical primary and metastatic models of colorectal cancer. I use flow cytometry and single-cell RNA seq to understand the tumor microenvironment. The anti-CD90.2 BUV563 antibody I used for the metastatic tumor analysis.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Spleen or Immune cells from any tissue

Primary Incubation

30 minute on Ice

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD Symphony A3

Results Summary

The result here is shown for CD90.2 population in spleen by ex vivo staining. After spleen cell isolation, they were stained with a fixable viability dye (Zombie UV) and various other antibodies antibodies. All steps are stained on ice for 20-30 mins. The result shown here is for CD90.2 population - live-gated CD45+CD11b-B220- . The Anti-CD90.2 BUV563 antibody was used at 1:400 dilution.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very good working dilution.

The Bad

N/A.

The Bottom Line

Very good antibody, I am very happy.

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