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PE anti-human HLA-DR, DP, DQ Antibody
361716
Tü39
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The goal of this research was to look for MHC presentation (in this case HLA DR, DP, DQ) after an activation-inducing treatment of CD14+ CD11c+ dendritic cells.
Flow cytometry
Human dendritic cells
0.1uL Zombie Green viability stain in 100uL PBS, incubated at room temp for 15 mins.
Human TruStain Fc block, 5uL diluted in 95uL FACS buffer (5% FBS in DPBS), incubated at 4 degrees C for 10 mins
1uL of HLA DR,DP, DQ antibody diluted in 100uL of FACS buffer (5% FBS in DPBS), incubated at 4 degrees C for 20 mins
Cytofix buffer, 100uL for 20 mins at 4 degrees C
Flow cytometry in a BD Celesta cytometer
This product helped us ensure that our treatment, which is meant to induce dendritic cell activation, increases MHC II presentation on the cell surface.
N/A
Stains well and inexpensive.
Overlap in the FITC channel- build panels with caution.
An inexpensive way to visualize an increase in MHC II (HLA DR, DP, DQ).