Great Antibody From Cell Signalling for Western Blot

October 04, 2023

Babraham Institute

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signalling

Product Name:

phospho S4281 (mTORC2)

Catalog Number:

2974

Clone Number:

Very clean blot from MCF10a cells, and easy to optimize. Would buy it again to detect pMTORC2.

Experimental Design and Results Summary

Applications

Western blot

Sample

MCF10a cells

Primary Incubation

1:1000 in 5% BSA overnight at 4 degrees

Blocking Agent

5% BSA

Secondary Incubation

1:1000 in 5% BSA for 1 hour at room temperature

Tertiary Incubation

N/A

Detection

ECL reagent

Results Summary

Very clean blot from MCF10a cells, and easy to optimise. Would definitely buy again. Don't need to use too much as it has high sensitivity.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use.

The Bad

Quite expensive.

The Bottom Line

Great signal, would recommend it.

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