Bio-Rad
Quick Start™ Bradford 1x Dye Reagent
500-0205
Info: Supplier Page
I routinely use this Bradford reagent from Bio-Rad for the estimation of protein quantity in my samples (cell lysates, cell supernatant, and biological fluids) before any end assay like western blot, biochemical assays, or ELISA.
Colorimetric
Cell lysate
Depending on the cells and their seeding density dilute the sample to get the concentration in the range of the standard curve.
Samples containing high quantity of protein showed dark purple color visually. All samples showed the absorbance in the range of BSA standard absorbance when read at 595 nm in spectrophotometer.
N/A
Easy and less time consuming method to quantify the proteins in the test samples.
Shows interference if the test sample have more than 4 M urea.
Good ready to use reagent for quick protein estimation.