Great Buffer From Stemcell Technologies Ideal for Isolating Monocytes From Peripheral Blood

UC Davis
Health Division of Gastroenterology

Overall

Quality of Results

Ease-of-Optimization

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Company:

STEMCELLTECHNOLOGIES

Product Name:

EasySep™ Buffer

Catalog Number:

20144

Info: Supplier Page 

We study circulating metabolites that regulate organismal physiology. We recently identified a few metabolites that are selectively induced upon infection in mice. We used this buffer to isolate monocytes from peripheral blood to test the effects of metabolites on monocytes in vitro.

Experimental Design and Results Summary

Application

Monocyte isolation from blood

Starting Material

Mice blood

Tips

Keep in 4 degrees C

Results Summary

We were able to isolate decent amount of monocytes from pooled blood from 5 mice and determined the pro-inflammatory effects of two metabolites.

DOI or PMID #

N/A

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Summary

The Good

Consistent quality.

The Bad

N/A

The Bottom Line

This is a great buffer for isolating monocytes from peripheral blood of mice and humans.

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