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Date Published
Filter Plate From Millipore Suitable for ELISPOT or MTS Assays
(1)
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MilliporeSigma
October 19, 2023
We use these plates to coat INF-y antibodies for ELISPOT or for cytotoxic assays. It is sturdy with deep wells for easy multi-channel ...
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Extraordinary Antibiotic From Thermo Fisher
(1)
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October 18, 2023
Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein ...
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Superb Reagent to Re-stimulate Human T-Cells
(1)
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Miltenyi Biotec
October 18, 2023
I used cytoStim reagent as a positive control to restimulate CD4+ and CD8+ T-cells present in PBMCs. After stimulation for 4-8h PBMCs were ...
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Cdkn2a Probe From ACD for the Accurate Detection of mRNA Levels
(1)
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October 18, 2023
We used p16 probe (Rn-Cdkn2a) to perform RNA-ISH in rat samples. Probe with ref. 423861 from ACD is great for detecting mRNA levels of ...
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Excellent Dye From Invitrogen for Nuclear Counterstain (DAPI)
(1)
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Thermo Fisher Scientific
October 18, 2023
DAPI is a water-soluble dye and emits blue fluorescence upon binding to AT regions of DNA which makes it a popular nuclear counterstain. ...
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Great Kit for Live/Dead Flow Calibration
(1)
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Thermo Fisher Scientific
October 18, 2023
We use these beads for live/dead compensation tubes. Works well with zombie green dye. We use one drop of reactive beads first, then add the...
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Reagent From Gibco Works Very Well to Dislodge Cells While Being Gentler Than Trypsin
(1)
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Thermo Fisher Scientific
October 17, 2023
Works very well to dislodge cells while being gentler than trypsin so we can still perform flow cytometry on them
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Satisfactory PCR Tubes From Applied Biosystems™
(1)
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October 17, 2023
MicroAmp Fast Reaction Tubes are highly durable and chemically resistant, making them ideal for PCR applications, as they can withstand ...
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DHEA Competitive ELISA Kit From Boster
(1)
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BosterBio
October 17, 2023
We used this kit to analyze saliva DHEA levels, with great success level. Validation of this assay has been straightforward. The assay ...
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Superb Kit From BioLegend for Live/Dead Cell Analysis
(1)
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BioLegend
October 17, 2023
Does a great job staining dead cells for accurate live/dead analysis in flow cytometry. Be sure to keep it in the dark, as it is light-...
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Antibody From StressMarq for Detecting P. falciparum HSP70 in Clinical Samples is Up to Standard
(1)
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StressMarq Biosciences Inc
October 17, 2023
We aimed to determine the level of pfHSP70, one of the Plasmodium falciparum chaperone proteins, in the peripheral blood of malaria patients...
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Decent Antibody From IBL for the Detection of IgAN
(1)
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IBL- America
October 17, 2023
Our group is developing personalized antibody-based treatments for IgA nephropathy. As such, we need specific markers for IgAN, to measure ...
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Thyroid Stimulating Hormone ELISA Kit From Boster Bio Works Well
(1)
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BosterBio
October 17, 2023
The purpose of this experiment was to quantify the level of TSH in a dried blood spot. The application of dried blood spots over whole blood...
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Great Anti-Human CD45 Antibody From BioLegend is Easy to Work With
(1)
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BioLegend
October 16, 2023
We are a cancer immunotherapy group, using cell-based approaches. Ultimately our products are tested in animal models, so good markers are ...
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Decent Anti-CD127 Antibody From BioLegend for the Flow Cytometric Detection of Memory T Cells
(1)
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BioLegend
October 16, 2023
The BV 421 CD127 antibody can be used for identifying memory CD8+ T cells within immune cell populations isolated from mouse lungs, spleen, ...
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Excellent Kit From Thermo Fisher for Visualizing Proliferating Mouse Cells at Any Stage
(1)
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Thermo Fisher Scientific
October 16, 2023
I used this kit to assess the cell proliferation status within the mouse's inner ear. This kit was very useful and worked well with no ...
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Excellent MUC18 Antibody From Abcam for Endothelial Cells
(1)
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Abcam
October 16, 2023
We needed to visualize cultured endothelial cells. MUC18 provides a great fluorescence for these cells.
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Great Reagent From Gibco Highly Recommended for Counting With No Contamination
(1)
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Thermo Fisher Scientific
October 16, 2023
I use this for sterile cell culture and counting. It doesn't harm the cells and never contaminates our culture. It also doesn't interfere ...
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Very Good CD64 Antibody From BioLegend
(1)
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BioLegend
October 16, 2023
Great CD64 antibody to use when staining intestine immune cells. Clear positive population, although the signal could be a bit brighter.
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Isolectin B4 From Vector Laboratories to Easily Stain the Endothelium
(1)
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October 13, 2023
Isolectin B4 was used to stain endothelial cells in heart samples obtained from rats. Staining is rapid, easy, and (almost) always works ...
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Anti-Human CD8 Antibody From BioLegend Works as Expected Giving High Quality Results
(1)
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BioLegend
October 13, 2023
I used this antibody to determine the percentage of CD8+ T cells in my expanded T cell population. This antibody generated a good signal ...
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Efficient High-fidelity DNA Polymerase From Invitrogen for Site-Directed Mutagenesis
(1)
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Thermo Fisher Scientific
October 13, 2023
We study how certain mutations affect viral replication. We construct our mutant cDNA infectious clones by PCR and this DNA polymerase mx is...
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CD14 MicroBeads From Miltenyi is an Easy and Reliable Way to Separate PBMCs
(1)
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Miltenyi Biotec
October 13, 2023
We use anti-human CD14 microbeads to purify monocytes and macrophages from human peripheral blood mononuclear cells (PBMC), as CD14 antigen ...
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Good Human IL-6 ELISA Kit From RayBiotech
(1)
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RayBiotech
October 12, 2023
Interleukin 6 (IL-6) is an inflammatory cytokine. I used a human IL-6 ELISA kit to detect levels of IL-6 in cell supernatant after 24 hours ...
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Excellent Reagent Mix From New England Labs for the Assembly of Large DNA Plasmids
(1)
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New England Biolabs
October 12, 2023
We study how different resistance mutations can affect Dengue virus replication. To do that, we need to obtain viral genetic material ...
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Highly Recommended Antibody From Cell Signaling for Western Blot
(1)
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Cell Signaling Technology
October 12, 2023
Clean blots, sensitivity is good, great for blotting for tubulin as a loading control. They are used in MCF10a cells.
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Great Cell Culture Reagent From Genesee
(1)
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Genesee Scientific
October 12, 2023
Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing ...
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Reliable Human IFN-gamma ELISA Kit From RayBiotech
(1)
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RayBiotech
October 12, 2023
IFN-gamma are known antivirals and are produced by immune cells in defense when one is exposed to the virus. I have used a Human IFN-gamma ...
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Collagenase Type VIII From Sigma Aldrich for Tissue Digestion
(1)
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MilliporeSigma
October 12, 2023
My research area includes studying the role of inflammation in the development of colorectal cancer. I often use different types of ...
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L-Glutamine From Thermo Fisher is Ideal to Culture HL-1 Cells
(1)
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Thermo Fisher Scientific
October 12, 2023
L-glutamine is diluted to 2 mM in Claycomb medium with 10% FBS, 1 % penicillin/streptomycin, and 0.1 mM noradrenaline and used for the ...
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Decent CD19 Antibody From BD Biosciences
(1)
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October 11, 2023
When used for flow cytometry on intestinal cells, there will be some background, but the B cell population is clear.
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A Good PAX6 Antibody From ThermoFisher Scientific
(1)
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Thermo Fisher Scientific
October 11, 2023
Our research focuses on understanding the role played by retina cells in neurodegenerative diseases. The specific goal of this experiment ...
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CellStar's 6-Well Cell Culture Plate is Ideal for Tissue Culture
(1)
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Greiner Bio-One GmbH
October 11, 2023
We put 1E6 dendritic cells per well which are adherent and grow very well and uniformly on the TC bottom. The plate is sturdy and never ...
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Straightforward, High Quality Anti-CD4 Antibody From BioLegend
(1)
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BioLegend
October 11, 2023
I used this antibody to determine what percentage of my T cell product was CD4+. I was able to quantify the subpopulation with no issues.
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Excellent Peroxidase-Labeled Antibody From Seracare for Focus Forming Assay
(1)
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October 11, 2023
I used this antibody as a secondary antibody in the focus-forming assay to determine the dengue virus titer and obtained satisfactory ...
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Great Cell Counting Slides From Logos Biosystems
(1)
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Logos Biosystems
October 11, 2023
Great for use in Luna Cell counter. We put 10uL samples in the end (9uL culture sample and 1uL stain). The slide has two ends so can be used...
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Great Anti-Albumin Antibody From Abcam for Rat Samples
(1)
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Abcam
October 10, 2023
Although Abcam does not indicate reactivity with rat samples, we conducted IHC-IF in the brain, and it worked perfectly well. Antigen ...
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Antibody From Invitrogen With Clear Signal But Was Average With My Cell Line
(1)
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Thermo Fisher Scientific
October 10, 2023
I used this antibody to attempt to determine B7H3 expression in a mouse tumor cell line and did not see any signal. Unfortunately, this ...
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Excellent Secondary Antibody From Invitrogen for IF
(1)
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Thermo Fisher Scientific
October 10, 2023
Goat anti-rabbit secondary antibody tagged with Alexa Fluor 488 is tested for immunofluorescence. Used at a concentration of 2.5 ug/ml for ...
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Good Protein Extraction Kit From Sigma Aldrich Requiring Protocol Modifications
(1)
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MilliporeSigma
October 10, 2023
The ProteoExtract Native Membrane Protein Extraction Kit is a valuable resource for membrane protein isolation; however, some protocol ...
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A Sturdy Flask From VWR With No Leaks Guaranteed
(1)
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VWR Scientific Products
October 10, 2023
This flask is great for cell culture. The holes on the lid provide gas exchange without allowing contamination. Has a good seal, and the ...
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Lamin B1 From Abcam for Rat Samples
(1)
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Abcam
October 10, 2023
We aimed to detect lamin B1 using rat brain samples, through IHC-IF. Overall, this antibody works fine.
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Mycoplasma Detection Kit From Lonza is Very Efficient
(1)
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Lonza
October 10, 2023
The MycoAlert® Mycoplasma Detection Kit is a vital tool for safeguarding the integrity of cell cultures, providing a user-friendly and ...
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Easily Accessible and Cheap TCR beta Antibody From BioLegend for Flow Cytometry
(1)
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BioLegend
October 09, 2023
The PerCPCy5.5 TCRbeta antibody can be used for identifying T cells within immune cell populations isolated from mouse lungs, spleen, and ...
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Great Reagent From Invitrogen for Flow Calibration
(1)
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Thermo Fisher Scientific
October 09, 2023
Provides great positive and negative populations for calibration before performing flow. Shows a great signal.
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Excellent Media From Corning for Cell Culture
(1)
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Corning Life Sciences
October 09, 2023
This media was used to culture cell amnion epithelial cells (AEC) in a study that investigated the induction of EMT in AECs in response to ...
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Cell Counter From Nexcelom Works Great!
(1)
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Revvity
October 09, 2023
The Spectrum Image Cytometer is a customizable cell counting and cytometry instrument that provides flow-like data with predesigned ...
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Good UV Excitable CD4 Antibody From BD Biosciences for CD4+ T Cell Identification
(1)
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October 09, 2023
The BUV496 CD4 antibody works well for distinguishing CD4+ T cells within immune cell populations isolated from mouse lungs, spleen, and ...
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Programmable ELISA Plate Washer From TriContinent
(1)
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October 09, 2023
We are doing routine ELISAs daily. This multi-washer allows for a more convenient routine, doing three wash cycles with ease along with ...
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Decent Cleaved Caspase-3 Antibody From Cell Signaling for Western Blot
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Cell Signaling Technology
October 09, 2023
I used this antibody several times for the detection of apoptosis induction in whole-cell lysates of prostate carcinoma cells. The detection...
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